Targeting SOX17 in Human Embryonic Stem Cells Creates Unique Strategies for Isolating and Analyzing Developing Endoderm
نویسندگان
چکیده
منابع مشابه
Targeting SOX17 in human embryonic stem cells creates unique strategies for isolating and analyzing developing endoderm.
Human embryonic stem cells (hESCs) can provide insights into development of inaccessible human tissues such as embryonic endoderm. Progress in this area has been hindered by a lack of methods for isolating endodermal cells and tracing fates of their differentiated progeny. By using homologous recombination in human ESCs, we inserted an enhanced green fluorescent protein (eGFP) transgene into th...
متن کاملExpression of Endoderm and Hepatic Specific Genes after in vitro Differentiation of Human Embryonic Stem Cells
Background: Human embryonic stem cells (hESC), which are derived from the inner cell mass of the blastocysts, have been considered to be pluripotent cells. In this study we examine the differentiating potential of hESC into hepatocytes by characterization of the expression of endoderm and liver-specific genes. Methods: hESC were cultivated in suspension to form aggregates, the embryoid bodies. ...
متن کاملInduction and selection of Sox17-expressing endoderm cells generated from murine embryonic stem cells.
Embryonic stem (ES) cells offer a valuable source for generating insulin-producing cells. However, current differentiation protocols often result in heterogeneous cell populations of various developmental stages. Here we show the activin A-induced differentiation of mouse ES cells carrying a homologous dsRed-IRES-puromycin knock-in within the Sox17 locus into the endoderm lineage. Sox17-express...
متن کاملstauprimide priming of human embryonic stem cells toward definitive endoderm
objective: in vitro production of a definitive endoderm (de) is an important issue in stem cell-related differentiation studies and it can assist with the production of more efficient endoderm derivatives for therapeutic applications. despite tremendous progress in de differentiation of human embryonic stem cells (hescs), researchers have yet to discover universal, efficient and cost-effective ...
متن کاملexpression of endoderm and hepatic specific genes after in vitro differentiation of human embryonic stem cells
background: human embryonic stem cells (hesc), which are derived from the inner cell mass of the blastocysts, have been considered to be pluripotent cells. in this study we examine the differentiating potential of hesc into hepatocytes by characterization of the expression of endoderm and liver-specific genes. methods: hesc were cultivated in suspension to form aggregates, the embryoid bodies. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cell Stem Cell
سال: 2011
ISSN: 1934-5909
DOI: 10.1016/j.stem.2011.01.017